Clonogenically Culturing and Expanding CD34+ Liver Cancer Stem Cells in Vitro.
...Published in Stem Cells and Development
A large number of cancer stem cells (CSCs) have been isolated and identified; however, none has been cultured in an unlimited manner in vitro without losing tumorigenicity and multipotency. In this study, we successfully clonogenically cultured a newly identified CD34+ liver CSC (LCSC) on feeder cells up to 22 passages (to date) without losing CSC ...
