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Clonogenically Culturing and Expanding CD34+ Liver Cancer Stem Cells in Vitro.

Park, Sc Zeng, C Tschudy-Seney, B Nguyen, Nt Eun, Jr Yan Zhang Ramsamooj, R Yan Zhang Min Zhao Theise, Nd ...

Published in Stem Cells and Development

A large number of cancer stem cells (CSCs) have been isolated and identified; however, none has been cultured in an unlimited manner in vitro without losing tumorigenicity and multipotency. In this study, we successfully clonogenically cultured a newly identified CD34+ liver CSC (LCSC) on feeder cells up to 22 passages (to date) without losing CSC ...

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