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Ion-selective self-referencing probes for measuring specific ion flux.

Brian Reid Min Zhao

Published in Communicative & Integrative Biology

The metal vibrating probe developed in the 1970s to measure electric current is sensitive down to the micro-Amp range, but detects only net current due to flow of multiple ions and is too large to measure from single cells. Electrophysiological techniques which use glass microelectrodes such as voltage clamping can be used on single cells but are a...

Ion-selective self-referencing probes for measuring specific ion flux

Brian Reid Min Zhao

Published in Communicative & Integrative Biology

Specific ion fluxes generate cornea wound electric currents.

Brian Reid Vieira, Ac Cao, L Mannis, Mj Schwab, Ir Min Zhao

Published in Communicative & Integrative Biology

The corneal epithelium generates a significant trans-epithelial potential (TEP) which aids in maintaining cornea water balance and transparency. Injury to the cornea causes a short circuit of the TEP at the wound. The TEP in the intact epithelium around the wound acts like a battery, powering significant ion flux and electric current at the wound. ...

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