Interaction between hedgehog signalling and PAX6 dosage mediates maintenance and regeneration of the corneal epithelium....
...Published in Molecular vision
Acanthamoeba migration in an electric field.
Published in Investigative Ophthalmology & Visual Science
Specific ion fluxes generate cornea wound electric currents.
Published in Communicative & Integrative Biology
The corneal epithelium generates a significant trans-epithelial potential (TEP) which aids in maintaining cornea water balance and transparency. Injury to the cornea causes a short circuit of the TEP at the wound. The TEP in the intact epithelium around the wound acts like a battery, powering significant ion flux and electric current at the wound. ...
Clonogenically Culturing and Expanding CD34+ Liver Cancer Stem Cells in Vitro.
...Published in Stem Cells and Development
A large number of cancer stem cells (CSCs) have been isolated and identified; however, none has been cultured in an unlimited manner in vitro without losing tumorigenicity and multipotency. In this study, we successfully clonogenically cultured a newly identified CD34+ liver CSC (LCSC) on feeder cells up to 22 passages (to date) without losing CSC ...
Electrical potential of acupuncture points: use of a noncontact scanning Kelvin probe.
Published in Evidence-based Complementary and Alternative Medicine
Objective. Acupuncture points are reportedly distinguishable by their electrical properties. However, confounders arising from skin-to-electrode contact used in traditional electrodermal methods have contributed to controversies over this claim. The Scanning Kelvin Probe is a state-of-the-art device that measures electrical potential without actual...
Beta-adrenergic receptor agonists delay while antagonists accelerate epithelial wound healing: evidence of an endogenous...
Published in Journal of Cellular Physiology
Wound healing is a complex and well-orchestrated biological process. Corneal epithelial cells (CECs) must respond quickly to trauma to rapidly restore barrier function and protect the eye from noxious agents. They express a high level of beta2-adrenergic receptors but their function is unknown. Here, we report the novel finding that they form part ...
Electric currents in Xenopus tadpole tail regeneration.
Published in Developmental Biology
Xenopus laevis tadpoles can regenerate tail, including spinal cord, after partial amputation, but lose this ability during a specific period around stage 45. They regain this ability after stage 45. What happens during this "refractory period" might hold the key to spinal cord regeneration. We hypothesize that electric currents at amputated stumps ...
An Experimental Model for Simultaneous Study of Migration of Cell Fragments, Single Cells, and Cell Sheets.
Published in Methods in Molecular Biology
Recent studies have demonstrated distinctive motility and responses to extracellular cues of cells in isolation, cells collectively in groups, and cell fragments. Here we provide a protocol for generating cell sheets, isolated cells, and cell fragments of keratocytes from zebrafish scales. The protocol starts with a comprehensive fish preparation, ...
Measurement of Bioelectric Current with a Vibrating Probe
Published in Journal of Visualized Experiments
The Electrical Response to Injury: Molecular Mechanisms and Wound Healing.
Published in Advances in Wound Care
Significance: Natural, endogenous electric fields (EFs) and currents arise spontaneously after wounding of many tissues, especially epithelia, and are necessary for normal healing. This wound electrical activity is a long-lasting and regulated response. Enhancing or inhibiting this electrical activity increases or decreases wound healing, respectiv...
